An Autochthonous Human Case of Fasciolopsiasis in Nepal

Fasciolopsiasis is rarely known as the parasitic disease in Nepal. Herein, we report a case of fasciolopsiasis in a 22-year-old man who was admitted in the hospital with abdominal pain, distension and loss of appetite for a month. He had previously diagnosed with acute viral hepatitis but, his abdominal pain was not resolving despite improvement in his liver function and general condition. During endoscopy an adult digenean worm was seen in the first part of the duodenum. After isolation, the worm was identified morphologically as Fasciolopsis buski. Microscogic examination of the patient’s stool revealed eggs with a morphology consistent with F. buski. Eggs were yellow-brown, ellipsoidal, unembmbryonated, operculated, filled with yolk cells, with thin shell and ranging 118–130 μm in length and 60–69 μm in width. The abdominal pain of the patient was resolved after treatment with praziquantel. By the present study, it was confirmed for the first time that fasciolopsiasis is indigenously transmitted in Nepal. Accordingly, the epidemiological studies in humans and reservoir host animals should be performed intensively in near future.

Fasciolopsiasis: Endemic focus of a neglected parasitic disease in Bihar.

Purpose: To describe a newly discovered, previously unreported endemic focus of fasciolopsiasis in the Phulwaria village, under tehsil Sugauli, East Champaran, Bihar. Materials and Methods: A study was conducted at village Phulwaria, following diagnosis of fasciolopsiasis in three children from the village. A total of 120 individuals, including all the children and adults who gave history of recent passage of red fleshy masses in their stool, were included in the study. The cases of fasciolopsiasis were treated with Praziquantel 25 mg/kg, three doses a day. Risk factors for the transmission of the parasite in the village were also studied. Results: Questionnaire revealed majority of the population suffering from abdominal discomfort and passage of red fleshy masses in stool. These fleshy masses were identified as Fasciolopsis buski. One hundred and eighteen individuals were presumably considered as cases of the parasitic infection. After treatment with Praziquantel, all of them passed the parasite in their stool for the next 2–3 days. On investigating, it was observed that all the conditions required for effective continuation of the life cycle of the parasite were present in this village. Conclusion: This study draws attention to a new endemic focus of fasciolopsiasis in Bihar, with a very high prevalence due to poverty, the lack of awareness about the parasite in villagers as well as ignorance among local medical practitioners. There is an urgent need for mass campaign around the region for its effective control.

Fasciolopsis buski Infection in a 50-Year-Old Female.

Fasciolopsiasis is a disease caused by the largest food-borne intestinal trematode known as Fasciolopsis buski. Here we report a case of a 50-year-old female who presented with upper abdominal pain and vomiting for one month. She was previously diagnosed as a case of choledocholithiasis. Endoscopic retrograde cholangio-pancreatography (ERCP) revealed stones along with two leaf-shaped worms which were removed from the duodenum of the patient. The worms were identified as F. buski by its unique morphology. Awareness regarding this parasitic infestation, especially in the rural area, should gain attention while formulating strategies to prevent and manage such infestation.

Fasciolopsiasis presenting as intestinal perforation: A case report.

Infestation by the zoonotic trematode Fasciolopsis buski (fasciolopsiasis) is seen in several parts of South-East Asia. Abdominal pain, diarrhoea, mucosal ulceration, intestinal obstruction, anasarca, and even fatality are described following heavy infestation. We present here the case of a 10-year-old boy from the Barabanki district of Uttar Pradesh, India with heavy infestation by Fasciolopsis buski causing intestinal perforation. Fasciolopsiasis is by no means rare but its presentation as a case of intestinal perforation is extraordinary.

Report on a fasciolopsis buski case detected in human in Nghe An province

A male patient of 2,5 year old living in Vinh city, Nghe An province, who often had abdominal paine. On October 3rd, 2001, he has vomitted with 8 flukes, which were identified by morphology and lately confirmed by molecular approach in Institute of Biological Technology as Fasciolopsis buski

Improved Staining Method for Permanent Specimen of Fasciolopsis buski / 中国寄生虫学与寄生虫病杂志

Fasciolopsis buski speciemens were collected, fixed with neutral formalin fixative solution, stained with alum-carmine staining solution, discolored with 2% kalium alum. The fixed and stained specimen shows clear internal structure with bright color, and can be stored for long time.

SPERMATOGENESIS IN FASCIOLOPSIS BUSKI / 重庆医科大学学报

In this study the process of spermatogenesis of Fasciolopsis buski has been observed by using the air-drying method.The results show that F. buski has the same process of spermatogenesis as that of the other digeneans. After 3 times of mitotic divisions as well as first and second meiotic divisions the spermid atage(32-cell stage) is formed through the process of the primary spermatogonial stage (1-cell stage), secondary and tertiary spermatogonia (2 - and 4-cell stages), primary and secondary spermatocytes (8 - and 16-cell stages). The spermatid then matures into 32 spermatozoa.The metaphase of spermatogonia has 14 chromosomes, which appear as "X" and "-" shapes, the metaphase I has 7 bivalent chromosomes, which form 7co-shaped con-figurations, while the metaphase II has 7 haploid chromosomes, which appear as "X" and"-" shapes, Therfore, they can be differentiated easily.Compared our results with that of Liao et al (1987), there are some differences in the descriptions of chromosomes of the metaphase I and II of meiosis, and of the metaphase of spermatogonia. The reason may be that Liao et al (1987) did not differentiate the metaphase I and II of meiosis

KARYOTYPE ANALYSIS OF FASCIOLOPSIS BUSKI / 重庆医科大学学报

In this study, the karyotype of Fasciolopsis buski has been investigated by the air drying method. The results show that the diploid number of chromosomes is 2n =14. The means and standard deviations of the relative lengths, arm indices and centromeric indices of the chromosomes have been calculated. According to the nomenclature recommended by Levan et al (1964), the karyotype of F. buski obtained consists of one pair of large-sized metacentrics (m), 2 pairs of medium-sized metacentrics (m), one pair of medium-sized submetacentrics (sm), one pair of small-sized metacentrics (m), one pair of small-sized submetacentrics (sm) and one pair of small-sized telocentrics (t).Idiogram of chromosomes has been constructed from measurements of 19 good metaphase spreads in mitoses.The karyotype of F. buski of this paper is compared with that reported by Gao et al (1952). Statistically, there are very distinct differences (p